RNAs for both CGRP and its receptor subunits were detected in the cerebellum of C57BL/6 mice.
CGRP immunoreactivity was detected in PCs and deep cerebellar nuclei.
EYFP was present in fastigial nucleus neurons after injection of AAV-EF1a-DIO-EYFP into calca-cre mice but wasn’t detected in PCs, which suggests that PCs accumulate, but do not produce CGRP.
Injection of CGRP to the fastigial nucleus induced light aversion, anxiety, and allodynia, while delivery to the vermis V induced anxiety and a trend towards light aversion.
Phenotypes began to present from different time points following injection of CGRP into vermis V and fastigial nucleus, suggesting that light aversion, as well as anxiety, was present.
Preliminary data showed that CGRP injection to the fastigial nucleus of L7/hRAMP1 mice and control littermates induced comparable light aversion and anxiety, suggesting that RAMP1 in PCs is not rate-limiting for those CGRP actions.
CGRP injection into crus I showed a trend to impair timing.