Findings

What does this study add?

• RNAs for both CGRP and its receptor subunits were detected in the cerebellum of C57BL/6 mice.
• CGRP immunoreactivity was detected in PCs and deep cerebellar nuclei.
• EYFP was present in fastigial nucleus neurons after injection of AAV-EF1a-DIO-EYFP into calca-cre mice but wasn’t detected in PCs, which suggests that PCs accumulate, but do not produce CGRP.
• Injection of CGRP to the fastigial nucleus induced light aversion, anxiety, and allodynia, while delivery to the vermis V induced anxiety and a trend towards light aversion.
• Phenotypes began to present from different time points following injection of CGRP into vermis V and fastigial nucleus, suggesting that light aversion, as well as anxiety, was present.
• Preliminary data showed that CGRP injection to the fastigial nucleus of L7/hRAMP1 mice and control littermates induced comparable light aversion and anxiety, suggesting that RAMP1 in PCs is not rate-limiting for those CGRP actions.
• CGRP injection into crus I showed a trend to impair timing.